Consistent, high quality RNA/DNA/Proteins ready for downstream applications; Sequentially isolate nucleic acids and proteins from a single lysate – no need to split the lysate; Isolate total RNA including microRNA; Proteins are eluted ready to use- require no further clean up or purification; No phenol or chloroform extractions; Fast and easy processing using vacuum manifold,centrifuge and easily automatable.
This 96-well kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast.The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing silicon carbide resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.The proteins are also purified on the second plate after RNA elution. The proteins are eluted in buffer and are ready for downstream applications without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry.