- ￥1180 - 1980
- Bioss
- bsm-34153M
- 2025年10月24日
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50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bsm-34153M |
| 英文名称 | NAPSIN A Mouse mAb |
| 中文名称 | 天冬氨酸蛋白酶ASP4抗体 |
| 英文别名 | NAPSIN A; Asp 4; ASP4; Aspartyl protease 4; KAP; Kdap; Kidney derived aspartic protease like protein; NAP1; NAPA; Napsa; NAPSA_HUMAN; Napsin 1; napsin A aspartic peptidase; Napsin A precursor; Napsin-1; Napsin-A; Pronapsin A; SNAPA; TA01/TA02. |
| 产品应用 | IHC-P=1:100-500, IF=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat, 肾, human lung carcinoma, human lung carcinoma, human lung carcinoma, human lung carcinoma, lung, lung, lung, lung, lung, lung, lung, human lung carcinoma, human lung carcinoma, human lung carcinoma |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse NAPSIN A |
| 亚型 | IgG1, Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 39 kDa |
| 浓度 | 1mg/ml |
| 储存液 | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300. |
| 亚细胞定位 | Secreted (Potential). |
| 组织特异性 | Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. |
| 相似性 | Belongs to the peptidase A1 family. |
| 功能 | May be involved in processing of pneumocyte surfactant precursors. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It is a 420-amino acid polypeptide consisting of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. The mature Napsin A protein contains 3 predicted disulfide bonds, 3 potential N-linked oligosaccharide attachment sites, an RGD motif, a recognition motif for integrin binding, in the C terminus, immediately before a 4-amino acid insert that is unique to aspartic proteinases. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IF} | {1:100-500} |
| {IHC-F} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (bsm-34153M) at 1:100overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (bsm-34153M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (bsm-34153M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (bsm-34153M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (bsm-34153M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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NAPSIN A Mouse mAb(bsm-34153M)-50ul/100ul
￥1180 - 1980
