Blank control（black line）:HepG2.
Primary Antibody (green line): Mouse Anti-CD81 antibody (bsm-51378M)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Mouse IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 1980
- Bioss
- bsm-51378M
- 2025年10月24日
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bsm-51378M |
| 英文名称 | CD81 Mouse mAb |
| 中文名称 | CD81小鼠单克隆抗体 |
| 英文别名 | 26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan-28; Tspan28. |
| 产品应用 | WB=1:500-5000, Flow-Cyt=1ug/Test, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD81 |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 26 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 |
Immunology > Adaptive Immunity > B Cells > CD Microbiology > Interspecies Interaction > Host Virus Interaction Microbiology > Protein > Human Protein > Receptor |
| 亚基 | Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Interacts with CD53 and SCIMP. |
| 亚细胞定位 | Membrane. |
| 组织特异性 | Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. |
| 翻译后修饰 | Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. |
| 相似性 | Belongs to the tetraspanin (TM4SF) family. |
| 功能 | May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-5000} |
| {Flow-Cyt} | {1ug/Test} |
| {ELISA} | {1:5000-10000} |
25 ug total protein per lane of various lysates (see on figure) probed with CD81 monoclonal antibody, unconjugated (bsm-51378M) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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CD81 Mouse mAb(bsm-51378M)-50ul/100ul
￥1180 - 1980
