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- Bioss
- bsm-43141M
- 2025年10月24日
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50ul/100ul/200ul/200ug
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-43141M |
| 英文名称 | CEACAM5 Mouse mAb |
| 中文名称 | 癌胚抗原单克隆抗体 |
| 英文别名 | Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, human lung carcinoma, human colon carcinoma, human colon carcinoma, human colon carcinoma, human colon carcinoma, esophagus, esophagus, esophagus, esophagus, stomach, stomach, stomach, stomach, colon, colon, colon, colon, appendix, appendix, appendix, appendix, Human esophageal cancer, Human esophageal cancer, Human esophageal cancer, Human esophageal cancer |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human CEACAM5 protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 77 kDa |
| 实际分子量 | 180-200kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers > Tumor antigens Cancer > Tumor immunology > Tumor-associated antigens Stem Cells > Mesenchymal Stem Cells > Surface Molecules Tags & Cell Markers > Cell Type Markers > Tumor Associated |
| 亚基 | Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor |
| 组织特异性 | Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. |
| 翻译后修饰 | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. |
| 相似性 | Belongs to the immunoglobulin superfamily. CEA family. |
| 功能 | Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:400-800} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (bsm-43141M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (bsm-43141M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (bsm-43141M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (bsm-43141M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human appendix); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (bsm-43141M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (bsm-43141M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Recombinant human CEACAM5 protein, C-His
Primary: Anti-CEACAM5 (bsm-43141M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 200,140 kDa
Lane 1: Human LOVO cell lysates
Lane 2: Recombinant human CEACAM5 protein, C-His
Primary: Anti-CEACAM5 (bsm-43141M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 200,140 kDa
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CEACAM5 ) Monoclonal Antibody, Unconjugated (bsm-43141M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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CEACAM5 Mouse mAb(bsm-43141M)-50ul/100ul/200ul/200ug
￥1180 - 5600
