Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60557M
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50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60557M |
| 英文名称 | Cyclin D3 Recombinant Mouse mAb |
| 产品应用 | WB=1:500-1000, ICC/IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat |
| 抗体来源 | Mouse |
| 亚型 | IgG1, Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Recombinant |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cyclins > Cyclin D family Cell Biology > Cell Cycle > Cyclins Cell Biology > Cell Cycle > Cyclins > Cyclin D Family Epigenetics and Nuclear Signaling > Cell cycle > Cyclins > Cyclin D Family |
| 亚基 | Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either CDKN1A or CDKN2A. |
| 亚细胞定位 | Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. |
| 组织特异性 | |
| 翻译后修饰 | Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation. |
| 相似性 | Belongs to the cyclin family. Cyclin D subfamily. Contains 1 cyclin N-terminal domain. |
| 功能 | Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |
| {ICC/IF} | {1:100-500} |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: BRL
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 33 kDa
Observed MW: 33 kDa
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: BRL
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 33 kDa
Observed MW: 33 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, Daudi
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 33 kDa
Observed MW: 33 kDa
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, Daudi
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 33 kDa
Observed MW: 33 kDa
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文献和实验相关实验
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
-4326) or cyclin D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20°C) cyclin
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20℃) cyclin staining
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
Immunodetection of cyclin D1 and D2 /D3 using 488/630 nm dual laser flow cytometry William Telford Hospital for Special Surgery
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Cyclin D3 Recombinant Mouse mAb(bsm-60557M)-50ul/100ul/25ul
￥800 - 2500
