Blank control: U2OS.
Primary Antibody (green line): Mouse Anti-RAB23 antibody (bsm-51516M)
Dilution: 1:50;
Isotype control antibody (blue line):Mouse IgG Secondary Antibody : Goat anti-mouse IgG-FITC
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 1980
- Bioss
- bsm-51516M
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Galectin 1 Rabbit pAb, PE conjugated(bs-6594R-PE)-100ul
￥2980
MDR1/P Glycoprotein Rabbit pAb, PE-Cy5 conjugated(bs-1468R-PE-Cy5)-100ul
￥2980
GPR89A Rabbit pAb(bs-15391R)-50ul/100ul/200ul
￥1180
Anti-human Complement C5 (Tesidolumab Biosimilar)(BIO0786SM)-1mg/5mg/20mg
￥1550
ROCK1 + ROCK2 Rabbit pAb(bs-10173R)-50ul/100ul/200ul
￥1180
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bsm-51516M |
| 英文名称 | RAB23 Mouse mAb |
| 中文名称 | G蛋白偶联受体RAB23单克隆抗体 |
| 英文别名 | DKFZp781H0695; HSPC137; MGC8900; Rab 23; RAB family small GTP binding protein RAB 23; RAB23, member RAS oncogene family; RAB23_HUMAN; Ras related protein Rab 23; Ras-related protein Rab-23. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1:50-100, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human RAB23 (Fragment) (His-tagged). |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 |
Neuroscience > Neurotransmission > Secretory Vesicles > Rabs Signal Transduction > Protein Trafficking > Vesicle Transport > Regulation Signal Transduction > Second Messenger > Nucleotide Messenger > GTP Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Ras Family Stem Cells > Signaling Pathways > Hedgehog > Surface Molecules |
| 亚基 | Interacts with SUFU. |
| 亚细胞定位 | Cell membrane; Lipid-anchor; Cytoplasmic side. Cell membrane. Cytoplasm. Cytoplasmic vesicle, autophagosome. Endosome membrane. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Note=Recruited to phagosomes containing S.aureus or M.tuberculosis. |
| 相似性 | Belongs to the small GTPase superfamily. Rab family. |
| 功能 | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies. Increasing data suggests an important role for Rab proteins in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. Rab 23, also known as HSPC137, is a 237 amino acid member of the Rab family of proteins and localizes to the cytoplasmic side of the cell membrane. Rab 23 is believed to play a role in intracellular protein transportation and signal transduction mediated by small GTPases. Mutations in the gene encoding Rab 23 may result in Carpenter syndrome, also known as ACPS2 (acrocephalopolysyndactyly type 2), a condition characterized by obesity, cardiac defects, polysyndactyly and craniosynostosis. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1:50-100} |
| {ELISA} | {1:5000-10000} |
Sample:
Lane 1: Mouse brain tissue lysates
Primary: Anti-RAB23 (bsm-51516M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Lane 1: Mouse brain tissue lysates
Primary: Anti-RAB23 (bsm-51516M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
. Use gloves and aerosol barrier tips. Linearize the plasmid and check the digest. Phenol extract. Extract twice with chloroform:isoamyl alcohol (24:1) Ethanol precipitate ( 1/2 vol 7.5 M NH4 OAc + 2.5 vol 100
Hybrid Resistance by Mouse NK Cells In Vitro
A purified NK cells to kill H2d target cells can be reversed by adding MAb reagents that bind to either Ly49A or H2-Dd . We and others have extended these early findings to the Ly49C and I subsets of NK cells, and have recently detected positive signaling
技术资料暂无技术资料 索取技术资料
RAB23 Mouse mAb(bsm-51516M)-50ul/100ul
￥1180 - 1980
