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50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60509R |
| 英文名称 | PKN1 Recombinant Rabbit mAb |
| 产品应用 | WB=1:500-1000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse) |
| 抗体来源 | Rabbit |
| 亚型 | IgG/Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > RNAi > Eukaryotic Initiation factors (eIF's) Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > S6K Family |
| 亚基 | Interacts (via N-terminus) with Rho1 (via REM repeats), Rac1 (via REM 1 repeat) and Rac2. |
| 亚细胞定位 | Cytoplasmic. |
| 组织特异性 | Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells. |
| 翻译后修饰 | Phosphorylated (By similarity). Autophosphorylated; autophosphorylation is stimulated by GTP-bound Rho/Rac GTPases. |
| 相似性 | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats. |
| 功能 | Pkc-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. May play a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion and transcription activation signaling processes (By similarity). Plays a role in regulating Rho-mediated dorsal closure during embryogenesis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |
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