Sample:
Lane 1: Spleen (Mouse) Tissue Lysate at 40 ug
Lane 2: HepG2 (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: BV-2 (Mouse) Cell Lysate at 30 ug
Primary:
Anti-phospho-Nrf2 (Ser40) (bs-23531R) at 1/1000 dilution
Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 120 kD
- ￥1180 - 2800
- Bioss
- bs-23531R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-23531R |
| 英文名称 | phospho-Nrf2 (Ser40) Rabbit pAb |
| 中文名称 | 磷酸化核因子2相关因子2(Ser40)抗体 |
| 英文别名 | NF2L2_HUMAN; Nuclear factor erythroid 2-related factor 2; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; Nrf-2; Nrf 2; Nuclear factor, erythroid derived 2, like 2; NFE2L2; NRF2; NFE2 like bZIP transcription factor 2; HEBP1; IMDDHH; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 68 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Cardiac metabolism Cell Biology > Other Antibodies > Oxidative Stress Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Transcription > Other factors Metabolism > Pathways and Processes > Metabolic signaling pathways > Nucleotide metabolism > Molecular processes > Mitochondrial transcription Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial Biogenesis Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress |
| 亚基 | Heterodimer. Forms a ternary complex with PGAM5 and KEAP1. May bind DNA with an unknown protein. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. |
| 亚细胞定位 | Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. |
| 组织特异性 | Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. |
| 翻译后修饰 | Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. |
| 相似性 | Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain. |
| 功能 | Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcriptionfactor which regulates the expression of many detoxification andantioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP) subfamily of basic /leucine zipper (bZIP) transcription factors. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 5.4 }}] {Qianyan Gao. et al. Ginsenoside Rg1 alleviates ANIT-induced cholestatic liver injury by inhibiting hepatic inflammation and oxidative stress via SIRT1 activation. J ETHNOPHARMACOL. 2024 Jan;319:117089} {IF} {Mouse}
[IF={{ 3.8 }}] {Liu Yujia. et al. Aerobic exercise improves BKCa channel-mediated vasodilation in diabetic vascular smooth muscle via AMPK/Nrf2/HO-1 pathway. ACTA DIABETOL. 2023 Dec;:1-10} {WB} {Mouse,Rat}
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：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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phospho-Nrf2 (Ser40) Rabbit pAb(bs-23531R)-50ul/100ul/200ul
￥1180 - 2800
