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- 规格:
50ul/100ul/25ul
| 规格: | 50ul | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2500.0 |
| 规格: | 25ul | 产品价格: | ¥800.0 |
| 产品编号 | bsm-60410M |
| 英文名称 | CD148 Recombinant Mouse mAb |
| 英文别名 | EC:3.1.3.48; Protein-tyrosine phosphatase eta; R-PTP-eta; Density-enhanced phosphatase 1; DEP-1; DEP1; HPTP eta; Protein-tyrosine phosphatase receptor type J (R-PTP-J); CD148 |
| 产品应用 | Flow-Cyt=1:50-200 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 亚型 | IgG1, Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Recombinant |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Phosphorylation > Tyrosine Kinases > Receptor Tyrosine Kinases Signal Transduction > Protein Phosphorylation > Tyrosine Phosphatases |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 酪氨酸磷酸酶,对CTNND1、FLT3、PDGFRB、MET、RET(变体MEN2A)、KDR、LYN、SRC、MAPK1、MAPK3、EGFR、TJP1、OCLN、PIK3R1和PIK3R2的去磷酸化起作用。在细胞粘附、迁移、增殖和分化中起作用。参与血管发育。巨噬细胞粘附和扩散的调节因子。积极影响细胞基质粘附。血小板活化和血栓形成的阳性调节因子。细胞增殖负调控因子。PDGF的负性调节因子刺激细胞迁移;通过PDGFR的去磷酸化。内皮细胞存活的正调节因子,以及VEGF诱导SRC和AKT活化的正调节因子;通过KDR去磷酸化。EGFR信号通路的负调节因子;通过EGFR去磷酸化。在重组过程中增强上皮连接的屏障功能。负性调节T细胞受体(TCR)信号。当T细胞TCR激活时,它被上调并排除在免疫突触之外,而当T细胞抗原呈递细胞(APC)脱离时,它不再被排除在外,并且可以去磷酸化PLCG1和LAT来下调信号的延长。 |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1:50-200} |
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Th17 Polarization of Mouse CD4 Cells
Anti-mouse CD28, clone37.51[Details: LEAF™format, cat.#102112] Anti-mouse IL-4 [Details: LEAF™format, cat#504108] Anti-mouse IFN-γ [Details: LEAF™format, cat.#505812] Recombinant mouse IL-6(carrier-free)[Details: Biolengend, cat
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
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