Sample:
Lane 1: Yeast lysates
Primary: Anti-Beta Actin (Yeast) (bs-8783R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
- ￥1180 - 1980
- Bioss
- bs-8783R
- 2025年10月24日
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-8783R |
| 英文名称 | Beta Actin (Yeast) Rabbit pAb, Loading Control |
| 中文名称 | 酵母肌动蛋白（内参）抗体 |
| 英文别名 | ACT_YEAST; Y-actin; Yeast Actin; ABY1; END7; β-actin; β actin; |
| 中文别名 | 酵母内参; |
| 产品应用 | WB=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Yeast |
| 抗体来源 | Rabbit |
| 免疫原 | Recombinant Yeast Actin. |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 42 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > NFkB pathway Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH Signal Transduction > Signaling Pathway > Nuclear Signaling > NFkB Pathway |
| 亚基 | Interacts with Rab6. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 翻译后修饰 | Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced. |
| 相似性 | Belongs to the actin family. |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
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[IF={{ 6.9 }}] {Zi-Long You. et al. Assembly and cooperative mechanism of the hexameric fungal plasma membrane H+-ATPase. CELL REP. 2025 Jun;44:115753} {WB} {}
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相关实验
Functional Characterization of Proteins Regulating Actin Assembly
Protocol 7: Measurements of the Treadmilling of Actin Filaments Support Protocol 1: Actin Purification from Rabbit Muscle Support Protocol 2: Preparation of Pyrenyl‐Labeled Actin Support
Yeast Chromatin Immunoprecipitation (ChIP)
Yeast Chromatin Immunoprecipitation (ChIP)Hahn LabJuly 2003Chromatin IP MethodDay 11.Start 5 ml o/n cultures of strains to be testedDay 21.Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o/n with shaking at 200
Use of Internaland External Standardsor Reference RNAs for A
levels of the gene under investigation are compared from sample to sample using an internal control to normalize for differences in sample concentration and loading. Ideally, the internal control should be a gene expressed at a constant level
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Beta Actin (Yeast) Rabbit pAb, Loading Control(bs-8783R)-50ul/100ul
￥1180 - 1980
