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PIK3R1 Mouse mAb(bsm-33219M)-5

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  • bsm-33219M
  • 2025年10月16日
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    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    规格：200ug产品价格：￥5600.0
    产品编号 bsm-33219M
    英文名称 PIK3R1 Mouse mAb
    中文名称 磷脂酰肌醇激酶单克隆抗体
    英文别名 P85A_HUMAN; Phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha(PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha); PI 3-kinase p85α; PI 3-kinase p85 α; PI 3-kinase p85-α; SH3_PI3K_p85alpha; PI3-kinase p85 subunit alpha; phosphoinositide-3-kinase regulatory subunit 1; p85; AGM7; IMD36; p85-ALPHA; PI 3 Kinase p85 alpha;
    产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.
    交叉反应 Human, Mouse, Rat (Pig)
    抗体来源 Mouse
    免疫原 Recombinant mouse PI3K p85 Protein
    亚型 IgG
    性状 Liquid
    纯化方法 affinity purified by Protein G
    克隆类型 Monoclonal
    理论分子量 80 kDa
    浓度 1mg/ml
    储存液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Signal transduction

    Immunology > Innate Immunity > TLR Signaling

    Signal Transduction > Signaling Pathway > Lipid Signaling > Lipid Kinases
    亚基 Heterodimer of a regulatory subunit PIK3R1 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with FER. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Interacts with PTK2/FAK1 (By similarity). Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with LYN (via SH3 domain); this enhances enzyme activity. Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C-terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1 and phosphorylated IRS4, as well as with NISCH and HCST. Interacts with FASLG, KIT and BCR. Interacts with AXL, FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with FGR and HCK. Interacts with PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with ERBB4 (phosphorylated). Interacts with NTRK1 (phosphorylated upon ligand-binding).
    亚细胞定位 Cytoplasmic.
    组织特异性 Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
    翻译后修饰 Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
    相似性 Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    功能 Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
    保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料 The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

     

    应用 推荐稀释比例
    {WB} {1:500-2000}
    {IHC-P} {1:100-500}
    {IHC-F} {1:100-500}
    {ICC/IF} {1:100-500}
    {IF} {1:100-500}

     

    产品细节图片1
    SH-SY5Y cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PI 3 Kinase p85 alpha) monoclonal Antibody, Unconjugated (bsm-33219M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片2
    Sample:
    Lane 1: Jurkat (Human) Cell Lysate at 30 ug
    Lane 2: NIH/3T3(Mouse) Cell Lysate at 30 ug
    Lane 3: Cerebrum (Mouse) Lysate at 40 ug
    Lane 4: Cerebrum (Rat) Lysate at 40 ug
    Lane 5: Heart (Mouse) Lysate at 40 ug
    Lane 6: Heart (Rat) Lysate at 40 ug
    Lane 7: Skeletal muscle (Mouse) Lysate at 40 ug
    Lane 8: Skeletal muscle (Rat) Lysate at 40 ug
    Lane 9: Spleen (Mouse) Lysate at 40 ug
    Lane 10: MOLT4 (Human) Cell Lysate at 30 ug
    Primary: Anti-PI 3 Kinase p85 alpha (bsm-33219M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 85 kD
    Observed band size: 85 kD
    Sample: BRL-3A Cell (Rat) Lysate at 40 ug
    Primary: Anti-PI3K p85 (bsm-33219M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 80 kD
    Observed band size: 80 kD
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3K p85) Monoclonal Antibody, Unconjugated (bsm-33219M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI 3 Kinase p85 alpha) Monoclonal Antibody, Unconjugated (bsm-33219M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 8.3 }}] {Jingjing Hu. et al. Raspberry ameliorates renal fibrosis in rats with chronic kidney disease via the PI3K/Akt pathway. PHYTOMEDICINE. 2025 May;140:156589} {WB} {Rat}

    [IF={{ 6.376 }}] {Xu, Qinqin. et al. Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice. MOL MED. 2022 Dec;28(1):1-14} {WB} {Mouse}

    [IF={{ 4.7 }}] {Xiao-Ying Gao. et al. Molybdenum interferes with MMPs/TIMPs expression to reduce the receptivity of porcine endometrial epithelial cells. CHEM-BIOL INTERACT. 2025 Jan;405:111304} {WB} {Porcine}

    [IF={{ 4.4 }}] {Liang Huang. et al. Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways. POULTRY SCI. 2024 Mar;:103696} {WB} {Broiler}

    [IF={{ 3.8 }}] {Xin Liu. et al. Role of PI3K/AKT signaling pathway involved in self-renewing and maintaining biological properties of chicken primordial germ cells. POULTRY SCI. 2024 Aug;:104140} {WB} {Chicken}

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    PIK3R1 Mouse mAb(bsm-33219M)-50ul/100ul/200ul/200ug
    ￥1180 - 5600