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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
12 months from date of receipt.
- 英文名:
Fluorescein hydrazide
- 库存:
99
- 供应商:
Biorbyt
- CAS号:
109653-47-6
- 规格:
5 mg/50 mg
| 规格: | 5 mg | 产品价格: | ¥0.0 |
|---|---|---|---|
| 规格: | 50 mg | 产品价格: | ¥0.0 |
描述: Fluorescein hydrazide is synthesized from dihydrazine adipate and fluorescein isothiocyanate as a colorimetric probe for aldehyde and ketone labeling. It is used in biochemistry and cell biology to study protein carbonylation in oxidative stress, applicable in both in vitro and in vivo research settings.
靶点: Others
分子式: C20H16N2O4
分子量: 348.35
SMILES: O=C(NN)C=1C=CC=CC1C2C3=CC=C(O)C=C3OC4=CC(O)=CC=C42
保存条件: -20°C
备注: For research use only.
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文献和实验Labeling of Oligonucleotides with Fluorescein
Oligonucleotides are a convenient alternative to conventional, cloned DNA probes since they can be rapidly synthesized in large quantities relatively cheaply. They can be made to very specific regions of the genome, without reference
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable
LABELING GC-GLOBULIN WITH FLUORESCEIN
Materials 1.2 mM PIPES,pH 7.0,500 ml. 2.Gc-globulin (Calbiochem 345802),1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine,100 mM in buffer 1,10 ml. 5.G25-150 desalting column,1 cm diameter.Equilibrated with buffer
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