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- 保存条件:
详询
- 保质期:
6-12个月
- 英文名:
4’-alpha-C-Azido-2’,3’-bis(O-t-butyldimethylsilyl)uridine
- 库存:
99
- 供应商:
Biorbyt
- 规格:
500 mg/100 mg/50 mg/25 mg/10 mg/5 mg
| 规格: | 500 mg | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 100 mg | 产品价格: | 询价 |
| 规格: | 50 mg | 产品价格: | 询价 |
| 规格: | 25 mg | 产品价格: | 询价 |
| 规格: | 10 mg | 产品价格: | 询价 |
| 规格: | 5 mg | 产品价格: | 询价 |
4’-alpha-C-Azido-2’,3’-bis(O-t-butyldimethylsilyl)uridine
产品名:4’-alpha-C-Azido-2’,3’-bis(O-t-butyldimethylsilyl)uridine
货号:orb1907659
产品中文名:4’-alpha-C-Azido-2’,3’-bis(O-t-butyldimethylsilyl)uridine
产品详情链接:https://www.biorbyt.com/4-alpha-c-azido-23-biso-t-orb1907659.html
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文献和实验Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
4 ‐thymidine cross‐link by solid‐phase synthesis in a (A ) directly opposing fashion and in a (B ) staggered fashion. (a) Oligonucleotide synthesis with 2′‐deoxyribonucleoside 3′‐phosphoramidites; (b) coupling with bis‐phosphoramidite 9 (labeled as T‐T ); (c
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