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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
- 保质期:
6个月
- 英文名:
Recombinant human Z DNA binding protein, N-His
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
20 ug
产品别名:C20orf183; DAI; DLM 1; DLM1; DNA-dependent activator of interferon regulatory factors; DNA-dependent activator of IRFs; OTTHUMP00000031373; OTTHUMP00000174290; OTTHUMP00000237854; Tumor stroma and activated macrophage protein DLM 1; Tumor stroma and activated macrophage protein DLM-1; Z-DNA-binding protein 1; ZBP 1; Zbp1; ZBP1_HUMAN; ZDBP1.
应用笔记:Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
纯度:>90% as determined by SDS-PAGE
蛋白序列:1-176/429
分子量:21.9 kDa
内毒素:Not analyzed
标签:N-His
浓度:>0.5mg/ml
Uniprot ID:Q9H171
Note:For research use only.

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文献和实验Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein
to avoid the time and expense of human tissue culture. This chapter focuses on the subcloning, purification, and initial functional validation of the recombinant human mitochondrial single-stranded DNA-binding protein. The cDNA encoding the mature form
重组蛋白所需要的代谢消耗,导致宿主菌生长的缓慢。低浓度下即影响宿主菌生长的基因产物被认为‘有毒’,这样的产物包括细胞膜蛋白或与DNA相互作用或干扰电子传递的蛋白。为了降低有毒蛋白在被诱导前对宿主菌生长的影响,在无诱导的时候的基础转录水平越低越好,并且诱导前的细菌传代数要控制在最小范围。细菌生长过程中质粒的丢失有时候可以通过在培养基中加入高浓度的ampicillin (200ug/ml) 或carbenicillin (50ug/ml)得到解决。对于一些不稳定的表达结构,应当避免过夜培养起始菌。先
Lambda gt11 cDNA libraries are routinely used for gene isolation with antibody and oligomer probes (1 –3 ). Briefly, a recombinant DNA library from different types of cells is constructed in a λgt 11 expression vector. The foreign peptides
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