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- biorbyt
- 英国
- orb1785265
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 保质期：
6个月
- 英文名：
Recombinant Vaccinia virus Envelope phospholipase F13 (F13L)
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：(37 kDa protein)(Envelope protein F13)(Palmitoylated EV membrane protein)(p37K)
应用笔记：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度：Greater than 90% as determined by SDS-PAGE.
生物来源：Vaccinia virus (strain Copenhagen) (VACV)
蛋白序列：MWPFASVPAGAKCRLVETLPENMDFRSDHLTTFECFNEIITLAKKYIYIASFCCNPLSTTRGALIFDKLKEASEKGIKIIVLLDERGKRNLGELQSHCPDINFITVNIDKKNNVGLLLGCFWVSDDERCYVGNASFTGGSIHTIKTLGVYSDYPPLATDLRRRFDTFKAFNSAKNSWLNLCSAACCLPVSTAYHIKNPIGGVFFTDSPEHLLGYSRDLDTDVVIDKLRSAKTSIDIEHLAIVPTTRVDGNSYYWPDIYNSIIEAAINRGVKIRLLVGNWDKNDVYSMATARSLDALCVQNDLSVKVFTIQNNTKLLIVDDEYVHITSANFDGTHYQNHGFVSFNSIDKQLVSEAKKIFERDWVSSHSKSLKI
分子量：49.3 kDa
表达区域：1-372aa
标签：N-terminal 10xHis-tagged and C-terminal Myc-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：E.coli
形态：Liquid or Lyophilized powder
Uniprot ID：P20638
Note：For research use only.
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文献和实验Isolation of Recombinant MVA Using F13L Selection
. This chapter describes the use of an efficient and easy-to-use selection system adapted for MVA. The system is based on the requirement of the viral gene F13L for efficient virus spread in cell culture, which results in a severe block in virus transmission
Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers
on plaque formation phenotype using the vaccinia virus gene F13L; the other relies on antibiotic resistance using the Escherichia coli xanthine-guanine phosphoribosyl transferase gene.
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
, 294]Dengue virus type 1 structural gene recombinant E proteinS, PHO1, prM virus signal sequence[295]Dengue virus type 2 envelope protein fusionI, 1 mg/50g cells; S, 100 mg/L, α-MF, functional553, 557Equine herpesvirus1 glycoprotein DS, 100 mg/L, α-MF
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