重组蛋白 Human Actin-related Prote
in蛋白 2/3 complex subunit 2 (ARPC2), partial，orb1096599，biorbyt- ￥3640
- biorbyt
- 英国
- orb1096599
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
Recombinant Human Actin-related protein 2/3 complex subunit 2 (ARPC2), partial
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：Arp2/3 complex 34KDA subunit , p34-AR, C
应用笔记：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度：Greater than 90% as determined by SDS-PAGE.
生物来源：Homo sapiens (Human)
蛋白序列：MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDY
分子量：55.5 kDa
表达区域：1-250aa
标签：N-terminal GST-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：E.coli
形态：Liquid or Lyophilized powder
Uniprot ID：O15144
Note：For research use only.
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文献和实验X-100或脱氧胆酸钠）和低浓度变性剂（2mol/L尿素或盐酸胍等）洗涤除去脂类和膜蛋白，这一步很重要，否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂，如8mol/L尿素、6~8mol/L盐酸胍，通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差，异氰盐酸胍最强；去污剂，如SDS[7]，可以破坏蛋白内的疏水键，可以增溶几乎所有的蛋白，但由于无法彻底去除而不允许用在制药行业中；酸，如70%甲酸[9]，可以破坏蛋白
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
. Bristow, J., Tee, M.K., Gitelman, S.E., Mellon, S.H., and Miller, W.L. 1993. Tenascin‐X: A novel extracellular matrix protein encoded by the human XB gene overlapping P450c21B. J. Cell. Biol. 122:265‐278
-based iterative library and fractions are collected. Eluates are screened for protein, and positive fractions generally contain a single protein, a small number of structurally related proteins, or a complex mixture. Only the first two categories
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