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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
6个月
- 英文名:
Human ACTB protein
- 库存:
99
- 供应商:
biorbyt
- 规格:
20 ug
产品别名:Beta-actin
应用笔记:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度:Greater than 85% as determined by SDS-PAGE.
生物来源:Homo sapiens (Human)
蛋白序列:MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
分子量:44.5 kDa
表达区域:1-375aa
标签:N-terminal 10xHis-tagged and C-terminal Myc-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:Baculovirus
形态:Liquid or Lyophilized powder
Uniprot ID:P60709
Note:For research use only.

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文献和实验qPCR引物设计,Primer3 Plus 和 Primerbank 都能搞定!
accession、NCBI protein accession、NCBI gene ID、primerbank ID、NCBI gene symbol 以及 keyword 六个选项进行搜索。以小鼠的 beta-actin 为例。1、首先,到 NCBI 上找出β-actin 的 gene ID,如图,选择 gene, 输入 beta-actin:点击右边的分类 musmusculus:结果就出来了,小鼠 beta-actin 的 NCBI gene ID 是 11461,而 actb 则是小鼠 beta
,其 gene symbol,也就是官方大名其实是叫 ACTB);在 Species 中选择物种 human or mouse;For text 中填入想查基因的 gene symbol;之后,Submit;可以看到针对 GAPDH 的不同对引物,具体序列,长度,Tm 值以及在其 cDNA 上位置等一些列信息点击「Click here for cDNA and amplicon sequence」,可以得到这对引物扩增的片段序列及位置:红色标记为上下游引物,蓝色标记为引物之间的扩增序列:超级方便有木
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
from the article "Human Housekeeping genes are compact," published in Trends in Genetics 19, 362-365 (2003). Each gene name/description is followed by its geometric average expression level according to the data published by Su et al. Genes designated by asterisk
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