- ￥3190 - 4301
- Biorbyt
- 1.57-100 ng/mL
- orb1817377
- 英国
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
Biorbyt
- 检测范围：
1.57-100 ng/mL
- 应用：
详询
- 适应物种：
Rat
- 样本：
serum, plasma and other biological fluids
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥4301.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥3190.0 |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Hepc 25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hepc 25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Hepc 25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Hepc 25 in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名：Rat Hepc 25(Hepcidin 25) ELISA Kit
货号：orb1817377
产品中文名：Rat Hepc 25(Hepcidin 25) ELISA Kit 酶联免疫试剂盒
产品详情链接：https://www.biorbyt.com/rat-hepc-25hepcidin-25-elisa-orb1817377.html
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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
of your primary antibody. 1) Heat Induced Epitope Retrieval Protocol 2) Based on the reagent that you are using, dilute the reagent to 1X as follows: To 25 ml 20×Citrate Buffer, pH 6.0 solution, add 475 ml distilled water
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
can destroy the protein. Use 25 µl of 0.41 mg/ml NGFNgf Standards ITube #NGF0.05% Acetic AcidFinal Conc.125 µl975 µl10 µg/ml2100 µl #19.9 ml100 ng/ml2.5100 µl #2900 µl10 ng/ml3100 µl #29.9 ml1 ng/mlUse the above solutions to make the following more dilute
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