Neptune Block ELISA Blocking B

DIRECT ELISA PROTOCOL

solution and wash the plate twice by filling the wells with 200 ?l PBS. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Blocking 4. Block the remaining

Sandwich ELISA Protocol

of 0.5 – 8 µg/ml in Coating Buffer and transfer 100 µl to each well of a high affinity, protein-binding ELISA plate. 2) Seal plate to prevent evaporation. Incubate at 4°C overnight. 2. Block the Plate: 1) Bring the plate

Testing Rabbit Bleeds By Elisa

and rinse wells 2Xs with TBST. Block 2 hr with 200 ul of 5 NFDM in TBST. Remove blocking reagent and rinse wells 2Xs with TBST. Incubate in primary antibody diluted in Blocking Buffer for 2 hr at RT. I do tripling dilutions beginning