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Mouse Glucoprotein 130 (gp130)

ELISA Kit试剂盒,orb1807934,biorbyt
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  • ¥3770
  • biorbyt已认证
  • 3.13-200ng/mL
  • 1.88 ng/mL
  • orb1807934
  • 英国
  • 2026年01月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      biorbyt

    • 检测范围

      3.13-200ng/mL

    • 检测方法

      Sandwich

    • 适应物种

      Mouse

    • 样本

      serum, plasma, Tissue homogenate and Other biological samples

    • 灵敏度

      1.88 ng/mL

    • 规格

      48 T

    产品描述:Mouse Glucoprotein 130 (gp130) ELISA Kit

    产品别名:IL6ST, CD13, CDW13, IL-6RB, IR6RB, Interleukin 6 signal transducer

    应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse gp130. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse gp130 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse gp130, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse gp130. You can calculate the concentration of Mouse gp130 in the samples by comparing the OD of the samples to the standard curve.

    实验时长:3.5H

    UniProt ID:Q00560

    靶点:gp130

    Note:For research use only.

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    图标文献和实验
    相关实验
    • Isolation and Culture of Mouse Germ Cells

      , targeted disruption of the signaling component of the LIF receptor, gp130 , demonstrated the importance of this signaling pathway for germ cell development in the embryo (2 ). Cultures of primordial germ cells (PGCs) have also been used as a model

    • Mouse Models for miRNA Expression: The ROSA26 Locus

      In 1991, Soriano and coworkers isolated the ROSA26 locus in a gene-trap mutagenesis screening performed in mouse embryonic stem (ES) cells. The ubiquitous expression of ROSA26 in embryonic and adult tissues, together with the high frequency

    • 组织解离的常见问题及答案

      Perfusion Kit, mouse and rat,130-128-030) 搭配gentleMACS Octo八通道带加热全自动组织解离器使用。   7. 如何得到心肌细胞? 小鼠心脏由多种类型细胞构成,包括心肌细胞、成纤维细胞、内皮细胞、免疫细胞、平滑肌细胞等。其中心肌细胞通过闰盘紧密连接,需要较强的酶解,但细胞膜脆弱,很容易在解离过程中破裂而造成细胞死亡。尤其是成年小鼠心肌细胞,体积大且不规则,更加大分离难度。 对于新生小鼠,闰盘未完全成熟,细胞连接没有十分紧密,可以使用常规解离方法,但可能出现得率

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