- ￥3770
- biorbyt
- 0.78-50ng/mL
- 0.47 ng/mL
- orb1807786
- 英国
- 2026年02月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.78-50ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.47 ng/mL
- 规格：
48 T
产品别名：ACP5，TRAP5b，SPENCDI
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TRACP-5b. You can calculate the concentration of Mouse TRACP-5b in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:Q05117
靶点：TRACP-5b
Note：For research use only.
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