- ￥3770
- biorbyt
- 0.16-10ng/mL
- 0.1 ng/mL
- orb1947469
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.1 ng/mL
- 规格：
48 T
产品别名：PLA2G4A, PLA2G4, cPLA2-alpha, cPLA2, phospholipase A2 group IVA
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human cPLA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human cPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human cPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human cPLA2. You can calculate the concentration of Human cPLA2 in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P47712
靶点：cPLA2
Note：For research use only.
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文献和实验Assaying Phospholipase A2 Activity
Mammalian cells contain many structurally and functionally diverse phospholipases A2 (PLA2 ) that catalyze the hydrolysis of sn-2 fatty acid from membrane phospholipid. Assays are described for measuring the activity of Group IVA cytosolic
formation (2 ) and specific eicosanoids seem to be produced by specific cell types in response to various inflammatory stimuli (3 ). The multiple synthetic pathways suggest that multiple sites of inhibition may exist. Phospholipase A2 (PLA2 ) acts on cell
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
