- ￥2800
- Tanda
- TD-26354
- 美国
- 2025年11月27日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉天德
- 库存：
999
- 靶点：
CTCF; MRD21
- 级别：
科研级
- 目录编号：
TD-26354
- 克隆性：
单克隆
- 抗原来源：
蛋白
- 保质期：
24个月
- 抗体英文名：
Anti-CTCF Mouse Monoclonal Antibody
- 抗体名：
CTCF Monoclonal Antibody
- 标记物：
FITC
- 宿主：
Mouse
- 适应物种：
脊椎动物
- 免疫原：
A synthetic peptide from the internal region of CTCF protein, human origin.
- 亚型：
IgG
- 形态：
液体
- 应用范围：
ELISA, WB, IHC
- 保存条件：
-20℃
- 浓度：
0.5 mg/ml
- 规格：
100μl
CTCF Monoclonal Antibody
| Cat. #: TD-26354 |
| Gene Symbol: CTCF; MRD21 |
| Description: Anti-CTCF Mouse Monoclonal Antibody |
| Background: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture. |
| Immunogen: A synthetic peptide from the internal region of CTCF protein, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 0.5 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes CTCF of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Purified His-tagged CTCF protein (lane 1) was blotted with anti CTCF monoclonal antibody (Cat. #TD-26354).
HEK293T cells was transfected with pCDNA3-GFP-CTCF plasmid, then fixed and stained with anti CTCF monoclonal antibody (Cat. #TD-26354). |
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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