- ￥3510
- biorbyt
- 1.25-80ng/mL
- 0.63 ng/mL
- orb1807551
- 英国
- 2026年02月06日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
1.25-80ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.63 ng/mL
- 规格：
48 T
产品别名：HEMB, P19, PTC, THPH8, Christmas Factor
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F9. You can calculate the concentration of Human F9 in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P00740
靶点：FGF9
Note：For research use only.
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，二者均为有包膜的正链RNA病毒。随后Simons用重组蛋白建立的ELISA技术检测非A-E肝炎病人的血清，发现一名西非病人血清抗GBV-A和GBV-B抗体反应阳性，用代表性差异分析扩增得到的为一个黄病毒样基因产物。该产物在核苷酸水平上与GBV-A，GBV-B和HCV的同源性分别为59％，47.9％和53.7％，而在氨基酸水平上同源性为64.2％，50.4％和57.3％。经检索发现，该基因序列与已发表且被公认的基因序列无任何同源性，因此将该序列所代表的病毒命名为GBV-C〔2〕。1995年美国Kim
颗粒都明显减少.设计的核酶构建了带有5’-、3’-自身修剪的真核细胞核酶表达质粒，包括含单个核酶和多个***的鸟枪型核酶基因的质粒，通过转染把他们同带有HBV基因组的质粒转入HepG2细胞. 用Southern blot、ELISA、RNase保护法等测定结果后，显示HBsAg、HBcAg、HBeAg以及他们相应的RNA、Dane颗粒数都因为核酶的作用而明显地减少[16]. Puthtz et al学者用重组筛选的方法从一个发夹型核酶文库中(5×109个)确定能有效地在转基因人肝癌细胞(HCC)中
