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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.31-20ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum
- 灵敏度:
0.16 ng/mL
- 规格:
48 T
产品别名:HO-1, HMOX1, HMOX1D, HSP32, Bk286b1
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HO1. You can calculate the concentration of Human HO1 in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P09601
靶点:HO1
Note:For research use only.
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文献和实验Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
demonstrated a specific upregulation of Hsp70 mRNA and protein after heat shock, while heme oxygenase 1 (HO1 or Hsp32) mRNA and protein was specifically upregulated following H2 O2 -induced oxidative stress. Hsp70 and HO1 should therefore be considered
: Heme oxygenase 1 mediates the immunomodulatory and antiapoptotic effects of interleukin 13 gene therapy in vivo in rats and in vitro in human cells. REFERENCE 59 (bases 1 to 1600) AUTHORS Lakkisto,P., Palojoki,E., Backlund,T., Saraste,A., Tikkanen
supernatant by a sandwich ELISA capturing apoB with a polyclonal goat anti-human apoB antibody (Chemicon International). apoB detection was performed with a horseradish peroxidase-conjugated goat anti-human apoB-100 polyclonal antibody (Academy Bio-Medical
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