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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
7.82-500pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
4.69 pg/mL
- 规格:
48 T
产品别名:IL8, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, 3-1C, AMCF-I, K6
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-8. You can calculate the concentration of Human IL-8 in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P10145
靶点:IL-8
Note:For research use only.
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文献和实验Mast Cell Activation by Lipoproteins
ribonucleic acid (mRNA) levels and protein levels of interleukin (IL)-8 an inflammatory cytokine. IL-8 is a potent chemoattractant for neutrophils and monocytes, which would result in a chronic inflammatory response. IL-8 mRNA levels were measured by reverse
A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
, including human interleukin (IL)-2, IL-4, IL-5, IL-6, IL-8, IL-13, IL-lβ, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α; mouse IL-4, IL-6, IFN-γ, and TNF-α; rat IL-2 and IFN-γ, and canine IFN-γ. Accumax was found to be compatible with human IL-13, IL
【申请上传】Current Protocols in Immunology
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