- ￥3770
- biorbyt
- 78.13-5000pg/mL
- 4.63 pg/mL
- orb1807301
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
78.13-5000pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
4.63 pg/mL
- 规格：
48 T
产品别名：NT3, NTF3, HDNF, NGF-2, NGF2
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NT-3. You can calculate the concentration of Human NT-3 in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P20783
靶点：NT3
Note：For research use only.
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文献和实验Visualization and Quantitation of Neurotrophin mRNAs
the possible existence of a gene family of related neurotrophic factors. The homologous sequences were used by several groups to isolate a third member of this neurotrophic factor family, neurotrophin-3 (NT-3) (Emfors et al., 1990a; Hohn
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Quantification of mRNA Levels Using Ribonuclease Protection Assay
to a region where its nucleotide sequence is fully conserved between the two species. Fig. 1. Generation of antisense RNA-probe and synthetic mRNA from plasmid construct. ( A ) An internal BglII-SacII fragment of the human NT-3
