- ￥3510
- biorbyt
- 3.13-200ng/mL
- 1.88 ng/mL
- orb1806833
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
3.13-200ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
1.88 ng/mL
- 规格：
48 T
产品别名：IgG
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgG. You can calculate the concentration of Rat IgG in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P20762
靶点：IgG
Note：For research use only.
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文献和实验Purification of Immunoglobulin G (IgG)
may consist of purification of total IgG or subpopulations (e.g., subclasses) of IgG from antisera/ascitic fluid/culture supernatant, or the isolation of a particular antigen binding fraction from such fluids. The former can be achieved by biochemical
是血清中免疫球蛋白主成分，约占血清中免疫球蛋白总含量的75％。其中40～50％分布于血清中，其余分布在组织中。分子量约为150000道尔顿。人类血清中的IgG主要为单体，正常人的IgG包括四个亚型，其IgG1 占60～70％，IgG2 占15～20％，IgG3 占5～10％，IgG4 占1～7％。这些亚型在补体激活的经典途中结合能力各不相同。IgG主要由脾脏和淋巴结中的浆细胞合成，是唯一能通过胎盘的抗体，对防止新生儿出生数周内的感染起很大作用。婴儿初生后第3个月已能合成
Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
The quantification of human immunoglobulin G (IgG) and recombinant derivatives is required for many applications in research and diagnostics. Particularly in research, robust and simple protocols are necessary to determine the concentration
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