- ￥3510
- biorbyt
- 15.63-1000pg/mL
- 5.47 pg/mL
- orb1807127
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000pg/mL
- 检测方法：
Competitive
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
5.47 pg/mL
- 规格：
48 T
产品别名：Corticotropin, Adrenocorticotropin
应用笔记：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human ACTH. During the reaction, Human ACTH in the sample or standard competes with a fixed amount of Human ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
实验时长：1.5H
UniProt ID:P63267
靶点：ACTH
Note：For research use only.
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Hormones as Biomarkers: Practical Guide to Utilizing Luminex Technologies for Biomarker Research
Hormones are chemical messengers produced in one part of the body and released into the blood to trigger or regulate particular functions of the body in another part. Hormone actions vary widely, but can include stimulation or inhibition
. One example of complex feedback occurs through the hypothalamic-pituitary target organ axis. Secretion of the hypothalamic corticotropin-releasing hormone releases pituitary ACTH, which in turn stimulates adrenal cortisol secretion. Subsequently
