- ￥3770
- biorbyt
- 0.31-20ng/mL
- 0.16 ng/mL
- orb1807147
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.31-20ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.16 ng/mL
- 规格：
48 T
产品描述：Human Versican/PG-M (VCAN) ELISA Kit
产品别名：CSPG2, VCAN, ERVR, PG-M, WGN, WGN1, Chondroitin Sulfate Proteoglycan 2, Versican Proteoglycan
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VCAN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VCAN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAN. You can calculate the concentration of Human VCAN in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P13611
靶点：VS
Note：For research use only.
产品别名：CSPG2, VCAN, ERVR, PG-M, WGN, WGN1, Chondroitin Sulfate Proteoglycan 2, Versican Proteoglycan
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VCAN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VCAN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAN. You can calculate the concentration of Human VCAN in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P13611
靶点：VS
Note：For research use only.
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，每一次循环所产生的DNA均能成为下一次循环的模板，每一次循环都使两条人工合成的引物间的DNA特异区拷贝数扩增一倍，PCR产物得以2n 的批数形式迅速扩增，经过25～30个循环后，理论上可使基因扩增109 倍以上，实际上一般可达106 ～107 倍。 假设扩增效率为“X”，循环数为“n”，则二者与扩增倍数“y”的关系式可表示为：y=(1+X)n 。扩增30个循环即n=30时，若X=100%，则y=230 =1073741824(>109 )；而若X=80%时，则y=1.830
Human Versican/PG-M (VCAN) ELISA Kit试剂盒，orb1807147，biorbyt
￥3770
