- ￥3900
- biorbyt
- 0.79-50 ng/mL
- 0.27 ng/mL
- orb1736668
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.79-50 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.27 ng/mL
- 规格：
48 T
产品别名：Vps34; Phosphatidylinositol 3-kinase p100 subunit; Phosphatidylinositol 3-kinase catalytic subunit type 3
应用笔记：standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIK3C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIK3C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIK3C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIK3C3 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:O88763
Note：For research use only.
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文献和实验【申请上传】Current Protocols in Immunology
Human B Cell Lines 1H Commonly Used Rat Strains 1I Peptide Binding Motifs for MHC Class I and II Molecules 1J Web Sites of Interest to Immunologists 1K Human MHC Genes and Products 1L Mouse MHC Genes and Products 1M Listing, Location, Binding
secondary antibody review -- data from 99 publications
ref host class subclass conjugation experiment dilution detail supplier 1 goat Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat Alexa Fluor 546 immunocytochemistry 1:250
