Thermal Shift Protein Stabilit

Methods for Protein Characterization by Mass Spectrometry, Thermal Shift (ThermoFluor) Assay, and Multiangle or Static Light Scattering

A recent development is the fluorescence-based thermal shift (ThermoFluor) assay. It uses an environmentally sensitive dye, Sypro Orange, to monitor the thermal stability of a protein and investigate factors (e.g., buffers, additives, and ligands) affecting

Introduction to the EMSA (Gel Shift) Technique

and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA). The EMSA technique is based on the observation that protein:DNA complexes migrate more slowly than free DNA molecules when subjected to non-denaturing polyacrylamide

Quantitative Determination of Protein Stability and Ligand Binding by Pulse Proteolysis

. Role of Calcium in the thermal stability of thermolysin. Biochemistry‐US 15:1103‐1111.    Gill, S.C. and von Hippel, P.H. 1989. Calculation of protein extinction coefficients