Thermal Shift Protein Stabilit

Methods for Protein Characterization by Mass Spectrometry, Thermal Shift (ThermoFluor) Assay, and Multiangle or Static Light Scattering

A recent development is the fluorescence-based thermal shift (ThermoFluor) assay. It uses an environmentally sensitive dye, Sypro Orange, to monitor the thermal stability of a protein and investigate factors (e.g., buffers, additives, and ligands) affecting

Introduction to the EMSA (Gel Shift) Technique

and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA). The EMSA technique is based on the observation that protein:DNA complexes migrate more slowly than free DNA molecules when subjected to non-denaturing polyacrylamide

The Combined Use of the Thermofluor Assay and ThermoQ Analytical Software for the Determination of Protein Stability and Buffer Optimization as an Aid

, also referred to as a thermal shift assay or differential scanning fluorescence (DSF), is a fast and simple method that is based upon high?throughput measurements of protein stability. The Thermofluor method can be performed on nearly all qPCR machines, meaning