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- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
15.60 - 1,000 pg/mL
- 适应物种:
Rat
- 样本:
Cell culture supernates, serum, and plasma.
- 灵敏度:
7 pg/mL
- 规格:
96 T
描述: The Rat β-NGF ELISA Kit features a dynamic range of 15.60 - 1,000 pg/mL and is suitable for Cell culture supernates, serum, and plasma. This product offers a sensitivity of 7 pg/mL.
别名: NGF; Beta-HSAN5; NGFB; nerve growth factor
研究领域: Neuroscience
保存条件: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.).
备注: For research use only.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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