Horse Tissue Inhibitors of Met

类别: Assays and Kits

描述: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 2(TIMP2).

别名: CSC-21K ELISA Kit, TIMP Metallopeptidase Inhibitor 2 ELISA Kit

应用说明: standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse TIMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TIMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse TIMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse TIMP2 in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间: 3.5h

研究领域: Musculoskeletal & Connective Tissue Research

UniProt ID: O77717

备注: For research use only.