Mmp12 Rabbit Polyclonal Antibody 抗体,orb1939847,Biorbyt产品图

Mmp12 Rabbit Polyclonal Antibo

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  • ¥5499
  • Biorbyt已认证
  • orb1939847
  • 英国
  • 2026年08月07日
  • ELISA, WB
  • Rabbit
  • Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Mmp12 Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      Mmp12 Rabbit Polyclonal Antibody

    • 靶点

      Macrophage metalloelastase

    • 浓度

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围

      ELISA, WB

    • 宿主

      Rabbit

    • 适应物种

      Mouse

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1939847

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Lyophilized

    • 亚型

      IgG

    • 免疫原

      详询

    • 规格

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Anti-Mmp12 Antibody. Tested in WB, ELISA applications. This antibody reacts with Mouse.

    别名: HME; Macrophage elastase; Macrophage metalloelastase; Matrix metalloproteinase 12; ME; MME; MMP 12; MMP12

    应用稀释比例: Western blot, 0.25-0.5 μg/ml, Mouse ELISA, 0.1-0.5 μg/ml

    免疫原: E.coli-derived mouse Mmp12 recombinant protein (Position: K237-H442). Mouse Mmp12 shares 62.7% and 90.3% amino acid (aa) sequence identity with mouse and rat Mmp12, respectively.

    交叉反应: No cross reactivity with other proteins.

    分子量: 45 kDa

    防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    纯化方法: Immunogen affinity purified.

    复溶/重构: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    研究领域: Signal Transduction

    UniProt ID: P34960

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of Mmp12 using anti-Mmp12 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mmp12 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mmp12 at approximately 45 kDa. The expected band size for Mmp12 is at 55 kDa.

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    图标文献和实验
    相关实验
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      Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed        

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

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