- ￥1560
- biorbyt
- orb1879771
- 英国
- 2025年12月04日
- FC, IF, IHC, WB
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
NDUFB4 Antibody抗体
- 抗体英文名：
NDUFB4 Antibody
- 应用范围：
FC, IF, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1879771
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human NDUFB4. The exact sequence is proprietary.
- 规格：
30 ul
产品描述：The NDUFB4 Antibody is suitable for FC, IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human NDUFB4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
别名:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; Complex I-B15; CI-B15; NADH-ubiquinone oxidoreductase B15 subunit
免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human NDUFB4. The exact sequence is proprietary.
应用稀释比例：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50), FC (1/10 - 1/30)
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:4710
UniProt ID:O95168
Note:For research use only.
别名:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; Complex I-B15; CI-B15; NADH-ubiquinone oxidoreductase B15 subunit
免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human NDUFB4. The exact sequence is proprietary.
应用稀释比例：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50), FC (1/10 - 1/30)
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:4710
UniProt ID:O95168
Note:For research use only.
Western blot analysis of NDUFB4 expression in HepG2 (A), PC3 (B), Ramos (C), U251MG (D) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 13 kD)
Immunohistochemical analysis of NDUFB4 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Anti-NDUFB4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Hela cells using Anti-NDUFB4 Antibody. The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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NDUFB4 Antibody抗体，orb1879771，biorbyt
￥1560
