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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit Goat IgG (H&L) - AP Antibody抗体
- 抗体英文名:
Rabbit Goat IgG (H&L) - AP Antibody
- 应用范围:
ELISA, ICC, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Goat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb1878902
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
AP
- 克隆性:
Polyclonal
- 形态:
1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% rAlbumin, 50% glycerol, 0.02% Sodium Azide
- 免疫原:
Goat IgG
- 规格:
100 ul
免疫原:Goat IgG
应用稀释比例:E (1/10000 - 1/50000), WB (1/5000 - 1/20000), IH (1/500 - 1/1000), IC (1/500 - 1/1000)
纯化:The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
保存说明:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:For research use only.
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文献和实验. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
5- add the AP conjugated tertiary antibody (50 to 100 µl) and incubate for 15 min. The tertiary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary antibody (Typically SBA goat anti rabbit-AP 1:100, rabbit anti goat-AP 1:300) 22- wash twice 23- [optional] add a tertiary layer (rabbit anti-goat-AP on a goat anti-rabbit-AP) 24- wash thrice 25- develop AP (see protocol
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