Goat Anti-Mouse/Rabbit IgG (H&

类别: Antibodies

产品子类型: Secondary Antibody

描述: The Goat Mouse/Rabbit IgG (H&L)-HRP Polymer (Ready to use) Antibody is suitable for ELISA, ICC, IHC, WB. It is a Polyclonal, HRP conjugated antibody which raised against Mouse/Rabbit IgG. Purification: Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

应用稀释比例: Ready to use

免疫原: Mouse/Rabbit IgG

防腐剂: PBS, pH 7.2, containing 1% rAlbumin, 0.05% Proclin

纯化方法: Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

特异性: By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.

研究领域: Cell Biology

保存条件: Shipped and store at 4°C for one year.

备注: For research use only.