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- Jena Bioscience
- 德国
- NU-821-X-CY3-L
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
- 保质期：
1年
- 英文名：
Aminoallyl-UTP-X-Cy3
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
无
氨基烯丙基-UTP-X-Cy 3，Aminoallyl-UTP-X-Cy3，氨基烯丙基-UTP-X-Cy 3,Aminoallyl-UTP-X-Cy3
氨基烯丙基-UTP-X-Cy 3-Aminoallyl-UTP-X-Cy3
产品货号：NU-821-X-CY3-L
产品规格：5x10ul(5mM)
Aminoallyl-UTP-X-Cy3; Nucleotides & Nucleosides;
Aminoallyl-UTP-X-Cy3; Nucleotides & Nucleosides;
氨基烯丙基-UTP-X-Cy 3-Aminoallyl-UTP-X-Cy3store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
点击：氨基烯丙基-UTP-X-Cy 3-Aminoallyl-UTP-X-Cy3查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Jena Bioscience公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验Preparation of probes for in situ hybridization
nucleotides, amino-allyl nucleotides are incorporated almost as well as unmodified UTP. Transcription reactions are then phenol extracted, RNA is ethanol precipitated, resuspended in 1 x SSC, and unincorporated nucleotides are removed by two rounds of gel
Preparation of probes for in situ hybridization
almost as well as unmodified UTP. Transcription reactions are then phenol extracted, RNA is ethanol precipitated, resuspended in 1 x SSC, and unincorporated nucleotides are removed by two rounds of gel filtration (1 x SSC buffered P30 micro-spin column, BioRad). RNA
) 20x SSC (175.5 mg/mL NaCl, 88.3 mg/mL NaCitrate) Cy3 and Cy5 Mono-reactive dye (Amersham PA23001 and PA25001): To 1 tube dye add 36 µL anyhdrous DMSO and divide into 16x 2µL aliquots Anhydrous DMSO: DMSO + Molecular Sieves
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