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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-Siglec-15 / CD33L3 Reference Antibody (NC318) 抗体
- 抗体英文名:
Anti-Siglec-15 / CD33L3 Reference Antibody (NC318)
- 浓度:
1mg/ml
- 应用范围:
ELISA, FA, FACS, Kinetics
- 适应物种:
Human, Mouse
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb1818066
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
MONOCLONAL
- 保存条件:
详询
- 形态:
Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
- 亚型:
IgG1
- 免疫原:
详询
- 规格:
100 ug
Anti-Siglec-15 / CD33L3 Reference Antibody (NC318)
产品名:Anti-Siglec-15 / CD33L3 Reference Antibody (NC318)
货号:orb1818066
产品中文名:Anti-Siglec-15 / CD33L3 Reference Antibody (NC318) 抗体
产品详情链接:https://www.biorbyt.com/anti-siglec-15-cd33l3-refere-orb1818066.html
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文献和实验The production of monoclonal antibody by hybridoma
. Keywords: monoclonal antibody ; hybridoma ; fusion Reference 1) Kohler G, and C. Milstein Continuous cultures of fused cells secreting
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
in goat serum 1 x 60' primary antibody (rabbit-anti-PAR-1 in 1% BSA, 10% serum in PBS) -- optionally incubated 4 hours @ 16 degrees C 1 x 10' wash in PBS 1 x 10' wash in PBS + 0.5% Tween 1 x 10' wash in PBS
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