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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
CKB Antibody / Creatine kinase B 抗体
- 抗体英文名:
CKB Antibody / Creatine kinase B
- 浓度:
详询
- 应用范围:
IHC-P
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb1825460
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
RECOMBINANT
- 保存条件:
Aliquot the recombinant CKBB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
- 形态:
详询
- 亚型:
Mouse IgG1, kappa
- 免疫原:
Recombinant human full-length protein was used as the immunogen for the recombinant CKBB antibody.
- 规格:
100 ug
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
产品名:CKB Antibody / Creatine kinase B
货号:orb1825460
产品中文名:CKB Antibody / Creatine kinase B 抗体
产品详情链接:https://www.biorbyt.com/ckb-antibody-creatine-kinase-orb1825460.html
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文献和实验Immunoprecipitation and Immune Complex kinase assay
9) Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning and resuspending. 10) If doing kinase assay, do additional wash in TN and then proceed with kinase assay (below). 11) Pellets can be stored dry
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
down bugs to which are bound the antibody and the antigen,aspirate supe,and resuspend by vortexing in 500 microliters lysis buffer. 9)Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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