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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-Creatine kinase B type / CKB Antibody
- 抗体英文名:
Anti-Creatine kinase B type / CKB Antibody
- 浓度:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
- 应用范围:
Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunohistochemistry (Frozen Only): 0.5-1ug/ml for 30 min at RT
- 宿主:
Mouse
- 克隆性:
/
- 保存条件:
≤-20°C
- 亚型:
IgG1, kappa
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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