- ￥9932
- Biorbyt
- orb1824167
- 英国
- 2025年07月07日
- ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Actin Antibody / Muscle Specific 抗体
- 抗体英文名：
Actin Antibody / Muscle Specific
- 浓度：
详询
- 应用范围：
ELISA
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1824167
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
RECOMBINANT
- 保存条件：
Aliquot the Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
- 形态：
详询
- 亚型：
Rabbit IgG, kappa
- 免疫原：
Recombinant human muscle actin protein was used as the immunogen for the Actin antibody.
- 规格：
100 ug
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
产品名：Actin Antibody / Muscle Specific
货号：orb1824167
产品中文名：Actin Antibody / Muscle Specific 抗体
产品详情链接：https://www.biorbyt.com/actin-antibody-muscle-specif-orb1824167.html
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
LABELING MUSCLE ACTIN WITH IATR
,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight
Labeling Muscle Actin with 5-iodoacetamidofluorescein
with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000},should be 0.5-0.9. 3.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Store as aliquots in liquid N2 after dissolving 2 mg sucrose per mg
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