- ￥3680 - 9936
- 卡梅德/KMD Bioscience
- 天津
- KMP2275
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
- 英文名：
Human Biotinylated ROR1 Protein, His Tag, Avi Tag
- 库存：
999
- 供应商：
卡梅德生物科技（天津）有限公司
- 规格：
20ug/100ug
| 规格： | 20ug | 产品价格： | ￥3680.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥9936.0 |
产品编号：KMP2275
产品名称：Human Biotinylated ROR1 Protein, His Tag, Avi Tag
中文名称： 生物素化重组人ROR1蛋白
中文全称：生物素化重组人受体酪氨酸激酶样孤儿受体蛋白
产品描述：产品描述：The Human Biotinylated ROR1 Protein(KMP2275) is produced in HEK293 Cells and the target gene encoding Gln30-Glu403 is expressed with a 6His, Avi tag at the C-terminus.
产品别称：受体酪氨酸激酶样孤儿受体, Inactive tyrosine-protein kinase transmembrane receptor ROR1, ROR1
分子名称：ROR1
预测分子量：42.82 kDa
物种：Human
宿主：HEK293 Cells
规格：50ug, 100ug, 200ug
纯化方式：Affinity purification
纯度：>95% as determined by SDS-PAGE
内毒素水平：<1.0 EU/ug determined by the LAL method
缓冲体系：PBS, pH7.4
Uniport:Q01973
功能：Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed:25029443). Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443, PubMed:27162350). In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed:27162350).产品背景：Receptor tyrosine kinase-like orphan receptor 1(ROR1), also known as neurotrophic tyrosine kinase, it is a member of the ROR family within receptor tyrosine kinases(RTK) superfamily. Human ROR1 is a type I transmembrane protein with 937 amino acids(aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain(ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. ROR1 expressed strongly in human heart, lung and kidney, but weakly in the CNS. At developmental stage, it expressed at high levels during early embryonic development. ROR1 has been shown to have very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling.
保存条件：Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
运输条件：In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
说明：This product is for research use only.
参考文献：1.PLoS ONE 9:E102695-E102695 (2014) 2.Proc. Natl. Acad. Sci. U.S.A. 113:5993-5998 (2016)
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文献和实验细胞表面的辅助受体CD4或CD8分子的胞浆尾部聚集，进而激活与胞浆尾部相连的酪氨酸激酶(Lck, Fyn和ZAP-70等)，促使CD3分子胞浆区的免疫受体酪氨酸活化基序(immunoreceptor tyrosine-based activation motif, ITAM)中的酪氨酸(Y)磷酸化。磷酸化的酪氨酸(pY)进一步磷酸化下游含酪氨酸的蛋白，从而引起激酶活化的级联反应(磷脂酰肌醇途径或MAP激酶途径等)，最终通过激活转录因子，使其进入细胞核内，结合于调控T细胞增殖和活化的靶基因(如IL-2和IFN
，进而激活与胞浆尾部相连的酪氨酸激酶(Lck, Fyn和ZAP-70等)，促使CD3分子胞浆区的免疫受体酪氨酸活化基序(immunoreceptor tyrosine-based activation motif, ITAM)中的酪氨酸(Y)磷酸化。磷酸化的酪氨酸(pY)进一步磷酸化下游含酪氨酸的蛋白，从而引起激酶活化的级联反应(磷脂酰肌醇途径或MAP激酶途径等)，最终通过激活转录因子，使其进入细胞核内，结合于调控T细胞增殖和活化的靶基因(如IL-2和IFN-g等)，引起基因的表达和转录，T
体(重组融合蛋白)中的蛋白A部分可与固相上抗原抗体复合物中的抗体IgG结合，而链亲合素部分可与生物素化的pUC19(质粒DNA) (Biotin-pUC19)中的生物素反应，从而将特定的DNA间接吸附于固相。接下来，就是第二步中的PCR过程，第一步中吸附于固相的pUC19质粒DNA在相应的引物存在下，可经PCR在几小时内而放大数百万倍，PCR产物的多少与固相上抗原的量成正比。 PCR由 ①待测抗原；②生物素化抗体；③亲和素（连接分子）；④生物素化DNA；⑤PCR扩增五部分构成。
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