- ￥1960
- BOSTER
- BA3110-2-100ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BA3110-2 |
|---|---|
| 产品名称 | Anti-ANXA6 Antibody |
| 基因名 | ANXA6 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 68-76KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VI(636-652aa NIRREFIEKYDKSLHQA), identical to the related mouse and rat sequences. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Annexin A6(ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca(2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization.The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal.Ca(2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion |
| 研究类别 | 1. Davies, A. A., Moss, S. E., Crompton, M. R., Jones, T. A., Spurr, N. K., Sheer, D., Kozak, C., Crumpton, M. J.The gene coding for the p68 calcium-binding protein is localized to bands q32-q34 of human chromosome 5, and to mouse chromosome 11.Hum. Genet. 82: 234-238, 1989.2. Smith, P. D., Davies, A., Crumpton, M. J., Moss, S. E.Structure of the human annexin VI gene.Proc. Nat. Acad. Sci. 91: 2713-2717, 1994.3. Thomas, D. D. H., Kaspar, K. M., Taft, W. B., Weng, N., Rodenkirch, L. A., Groblewski, G. E.Identification of annexin VI as a Ca(2+)-sensitive CRHSP-28-binding protein in pancreatic acinar cells.J. Biol. Chem. 277: 35496-35502, 2002. |
| Uniprot ID | ANXA6: P08133 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-ANXA6 antibody (BA3110-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANXA6 antigen affinity purified polyclonal antibody (BA3110-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANXA6 at approximately 68-76 kDa. The expected band size for ANXA6 is at 76 kDa.|Figure 2. Flow Cytometry analysis of HL-60 cells using anti-ANXA6 antibody (BA3110-2).
Overlay histogram showing HL-60 cells stained with BA3110-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANXA6 Antibody (BA3110-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human placenta tissue lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANXA6 antigen affinity purified polyclonal antibody (BA3110-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANXA6 at approximately 68-76 kDa. The expected band size for ANXA6 is at 76 kDa.|Figure 2. Flow Cytometry analysis of HL-60 cells using anti-ANXA6 antibody (BA3110-2).
Overlay histogram showing HL-60 cells stained with BA3110-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANXA6 Antibody (BA3110-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-Annexin A6（Annexin VI） Antibody(BA3110-2-100ul)
￥1960
