- ￥1180
- BOSTER
- BA3110-2-50ul
- 中国
- 2025年07月08日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | BA3110-2 |
|---|---|
| 产品名称 | Anti-ANXA6 Antibody |
| 基因名 | ANXA6 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 68-76KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VI(636-652aa NIRREFIEKYDKSLHQA), identical to the related mouse and rat sequences. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Annexin A6(ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca(2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization.The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal.Ca(2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion |
| 研究类别 | 1. Davies, A. A., Moss, S. E., Crompton, M. R., Jones, T. A., Spurr, N. K., Sheer, D., Kozak, C., Crumpton, M. J.The gene coding for the p68 calcium-binding protein is localized to bands q32-q34 of human chromosome 5, and to mouse chromosome 11.Hum. Genet. 82: 234-238, 1989.2. Smith, P. D., Davies, A., Crumpton, M. J., Moss, S. E.Structure of the human annexin VI gene.Proc. Nat. Acad. Sci. 91: 2713-2717, 1994.3. Thomas, D. D. H., Kaspar, K. M., Taft, W. B., Weng, N., Rodenkirch, L. A., Groblewski, G. E.Identification of annexin VI as a Ca(2+)-sensitive CRHSP-28-binding protein in pancreatic acinar cells.J. Biol. Chem. 277: 35496-35502, 2002. |
| Uniprot ID | ANXA6: P08133 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大Lane 1: human placenta tissue lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANXA6 antigen affinity purified polyclonal antibody (BA3110-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANXA6 at approximately 68-76 kDa. The expected band size for ANXA6 is at 76 kDa.|Figure 2. Flow Cytometry analysis of HL-60 cells using anti-ANXA6 antibody (BA3110-2).
Overlay histogram showing HL-60 cells stained with BA3110-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANXA6 Antibody (BA3110-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
Biology International''31(6):638-644. 货号3011，产品描述：Goat anti-Rabbit IgG Secondary antibody unconjugated； 货号3012，产品描述：Goat anti-Rabbit IgG Secondary antibody HRP conjugated； 货号3021，产品描述：Goat anti-Human IgG
Anti-c-myc-HRP生物素化分子μMACS Streptavidin Kit 免疫沉淀μMACS Protein A MicroBeadsμMACS Protein G MicroBeads 转染细胞MACSelect Kk KitMACSelect 4 KitMACSelect LNGFR KitAnti-Mouse H-2KkCD4-FITCCD271(LNGFR)-FITCMACSelect Control FITC Antibody 凋亡和死亡细胞分选与分析 细胞类别MACS分选产品
而数目相应的增加． (3)Annexin VI是一种在老鼠肝脏细胞中对肝生理活动起重要作用的蛋白质，经过双重胶体金标记老鼠肝脏超薄切片上的抗annexin VI抗体可定量检测annexin VI，标记效率是80％．这样annexin VI成为第一个已知的参与肝细胞受体循环和转运的结构蛋白质． (4)通过胶体金免疫电镜分析兔子十二指肠上皮细胞不同位置处的胃动素和血液中的复合胺表明，与复合胺反应的胶体金集中在分泌腺的颗粒上，而与胃动素反应的胶体金在颗粒外部，表明这两种物质
技术资料暂无技术资料 索取技术资料
