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- BOSTER
- A13973-1-100ul
- 中国
- 2025年07月16日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A13973-1 |
|---|---|
| 产品名称 | Anti-NECAB1 Antibody |
| 基因名 | NECAB1 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 41KD |
| 免疫原 | E.coli-derived human NECAB1 recombinant protein (Position: E16-E296). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | NECAB1, also named as EFCBP1, is a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing EF-hand and antibiotic biosynthesis monooxygenase domains Short Communication. |
| 研究类别 | 1. Wu, H. , Li, D. , Shan, Y. , Wan, B. , Hexige, S. , & Guo, J. , et al. (2007). Efcbp1/necab1, a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing ef-hand and antibiotic biosynthesis monooxygenase domains. Dna Seq, 18(1), 73-79.2. Zhang, M. D. , Barde, S. , Szodorai, E. , Josephson, A. , Mitsios, N. , & Watanabe, M. , et al. (2016). Comparative anatomical distribution of neuronal calcium-binding protein (necab) 1 and -2 in rodent and human spinal cord. Brain Structure & Function, 221(7), 1-21.3. Sugita, S. , Ho, A. , & T.C. Südhof. (2002). Necabs: a family of neuronal ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern. Neuroscience, 112(1), 51-63. |
| Uniprot ID | NECAB1: Q8N987 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Direct ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-NECAB1 antibody (A13973-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NECAB1 antigen affinity purified polyclonal antibody (A13973-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NECAB1 at approximately 41 kDa. The expected band size for NECAB1 is at 41 kDa.|Figure 2. IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in a paraffin-embedded section of human glioblastoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 5. IF analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in an immunocytochemical section of CACO-2 cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. [/list_product_images]
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NECAB1 antigen affinity purified polyclonal antibody (A13973-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NECAB1 at approximately 41 kDa. The expected band size for NECAB1 is at 41 kDa.|Figure 2. IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in a paraffin-embedded section of human glioblastoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 5. IF analysis of NECAB1 using anti-NECAB1 antibody (A13973-1).
NECAB1 was detected in an immunocytochemical section of CACO-2 cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. [/list_product_images]
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Anti-NECAB1 Antibody(A13973-1-100ul)
￥1960
