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- BOSTER
- PB0308-50ul
- 中国
- 2025年07月15日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB0308 |
|---|---|
| 产品名称 | Anti-LRRK2 Antibody |
| 基因名 | LRRK2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 286KD |
| 免疫原 | E.coli-derived human LRRK2 recombinant protein (Position: E10-L350). Human LRRK2 shares 82% amino acid (aa) sequence identity with mouse LRRK2. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is anenzyme that in humans is encoded by the PARK8 gene. It is a member of the leucine-rich repeat kinase family. This gene is mapped to 12q12. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. It has been determined that LRRK2 possesses mixed-lineage kinase activity, and LRRK2 also showed autophosphorylation activity. What’s more, LRRK2 has an affinity for lipids or lipid-associated proteins and may play a role in the biogenesis or regulation of membranous intracellular structures in the brain. It is also an IFNG target gene that may be involved in signaling pathways relevant to Crohn disease. |
| 研究类别 | 1. Biskup, S., Moore, D. J., Celsi, F., Higashi, S. West, A. B., Andrabi, S. A., Kurkinen, K., Yu, S.-W., Savitt, J. M., Waldvogel, H. J., Faull, R. L. M., Emson, P. C., Torp, R., Ottersen, O. P., Dawson, T. M., Dawson, V. L. Localization of LRRK2 to membranous and vesicular structures in mammalian brain. Ann. Neurol. 60: 557-569, 2006.2. Paisán-Ruíz C, Jain S, Evans EW, Gilks WP, Simón J, van der Brug M, López de Munain A, Aparicio S, Gil AM, Khan N, Johnson J, Martinez JR, Nicholl D, Carrera IM, Pena AS, de Silva R, Lees A, Martí-Massó JF, Pérez-Tur J, Wood NW, Singleton AB (November 2004). "Cloning of the gene containing mutations that cause PARK8-linked Parkinson's disease". Neuron 44 (4): 595–600.3. West, A. B., Moore, D. J., Biskup, S., Bugayenko, A., Smith, W. W., Ross, C. A., Dawson, V. L., Dawson, T. M. Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity. Proc. Nat. Acad. Sci. 102: 16842-16847, 2005. |
| Uniprot ID | LRRK2: Q5S007 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of LRRK2 using anti-LRRK2 antibody (PB0308). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Brain Tissue Lysate,Lane 3: Rat Liver Tissue Lysate,Lane 4: U87 Whole Cell Lysate,Lane 5: NEURO Whole Cell Lysate,Lane 6: A549 Whole Cell Lysate,Lane 7: SMMC Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRK2 antigen affinity purified polyclonal antibody (Catalog # PB0308) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRK2 at approximately 286KD. The expected band size for LRRK2 is at 286KD.[/list_product_images]
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Anti-LRRK2 Antibody(PB0308-50ul)
￥1180
