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大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | A02660-2 |
|---|---|
| 产品名称 | Anti-Serine racemase/SRR Antibody |
| 基因名 | SRR |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 免疫原 | E.coli-derived human Serine racemase/SRR recombinant protein (Position: A3-E283). |
| 内容 | 500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | SRR(serine racemase) is a 37 kDa protein and belongs to the serine/threonine dehydratase family. It plays a role in neuronal activity and a potential biological role in the regulation of N-methyl-D-aspartate (NMDA) receptor activity in these peripheral organs as well. SRR catalyzes the racemization between l- and d-serine. |
| 研究类别 | 1. De Miranda, J., Santoro, A., Engelender, S., Wolosker, H. Human serine racemase: molecular cloning, genomic organization and functional analysis. Gene 256: 183-188, 2000. 2. Henneberger, C., Papouin, T., Oliet, S. H. R., Rusakov, D. A. Long-term potentiation depends on release of D-serine from astrocytes. Nature 463: 232-236, 2010. 3. Labrie, V., Fukumura, R., Rastogi, A., Fick, L. J., Wang, W., Boutros, P. C., Kennedy, J. L., Semeralul, M. O., Lee, F. H., Baker, G. B., Belsham, D. D., Barger, S. W., Gondo, Y., Wong, A. H. C., Roder, J. C. Serine racemase is associated with schizophrenia susceptibility in humans and in a mouse model. Hum. Molec. Genet. 18: 3227-3243, 2009. |
| Uniprot ID | SRR: Q9GZT4 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- Serine racemase/SRR antibody (A02660-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates.
Use rabbit anti- Serine racemase/SRR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Serine racemase/SRR at approximately 37KD. The expected band size for Serine racemase/SRR is at 37KD.|Figure 2. Flow cytometry analysis of U251 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human 293T whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates.
Use rabbit anti- Serine racemase/SRR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Serine racemase/SRR at approximately 37KD. The expected band size for Serine racemase/SRR is at 37KD.|Figure 2. Flow cytometry analysis of U251 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-Serine racemase/SRR Antibody(A02660-2-100ul)
¥1960







